CoraLite® Plus 488-conjugated ZFP36 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZFP36 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5E6

Cat no : CL488-66938

Synonyms

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Validation Data Gallery

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  WB analysis using CL488-66938

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66938 (ZFP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NCI-H1299 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66938 targets ZFP36 in WB applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZFP36 fusion protein Ag28473
• Full Name: zinc finger protein 36, C3H type, homolog (mouse)
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009693
• Gene Symbol: ZFP36
• Gene ID (NCBI): 7538
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).