ZFP36 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZFP36 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A5E6

Cat no : 66938-1-Ig

Synonyms

G0S24, GOS24, NUP475, Protein TIS11A, RNF162A, TIS11, TIS11A, Tristetraproline, TTP, Zfp 36, ZFP36, Zinc finger protein 36 homolog

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 66938-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66938-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 66938-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66938-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 66938-1-Ig

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig lung using 66938-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, NCI-H1299 cells, LPS treated U-937 cells, 3T3-L1 cells, pig lung tissue, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

66938-1-Ig targets ZFP36 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZFP36 fusion protein Ag28473
• Full Name: zinc finger protein 36, C3H type, homolog (mouse)
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009693
• Gene Symbol: ZFP36
• Gene ID (NCBI): 7538
• RRID: AB_2882262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).

Publications

Species	Application	Title
human	WB,IF,IP	Cell Rep; A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma; Authors - Yue Li
human	WB	Int Immunopharmacol; Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.; Authors - Kaiyong Yang ; KD Validated