ZFP36 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ZFP36 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5E6

Cat no : 66938-1-PBS

Synonyms

G0S24, GOS24, NUP475, Protein TIS11A, RNF162A, TIS11, TIS11A, Tristetraproline, TTP, Zfp 36, ZFP36, Zinc finger protein 36 homolog

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Validation Data Gallery

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  WB analysis of HepG2 using 66938-1-Ig (same clone as 66938-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66938-1-Ig (same clone as 66938-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 66938-1-Ig (same clone as 66938-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66938-1-Ig (same clone as 66938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 3T3-L1 using 66938-1-Ig (same clone as 66938-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 66938-1-Ig (same clone as 66938-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66938-1-PBS targets ZFP36 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZFP36 fusion protein Ag28473
• Full Name: zinc finger protein 36, C3H type, homolog (mouse)
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009693
• Gene Symbol: ZFP36
• Gene ID (NCBI): 7538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).