ZEB2 Monoclonal antibody, PBS Only

ZEB2 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3A2

Cat no : 67514-1-PBS

Synonyms

FLJ42816, KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

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Validation Data Gallery

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  WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 67514-1-Ig (same clone as 67514-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67514-1-Ig (same clone as 67514-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67514-1-Ig (same clone as 67514-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67514-1-Ig (same clone as 67514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67514-1-Ig (ZEB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67514-1-Ig (same clone as 67514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67514-1-Ig (ZEB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67514-1-Ig (same clone as 67514-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ZEB2 Monoclonal antibody (67514-1-Ig, Clone:1D3A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67514-1-PBS targets ZEB2 in WB, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZEB2 fusion protein Ag25477
• Full Name: zinc finger E-box binding homeobox 2
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC127102
• Gene Symbol: ZEB2
• Gene ID (NCBI): 9839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )