ZEB2 Monoclonal antibody

• Featured Product
• KD/KO Validated

ZEB2 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1D3A2

Cat no : 67514-1-Ig

Synonyms

SIP1, Smad-interacting protein 1, SIP 1, KIAA0569, HRIHFB2411

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Validation Data Gallery

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  WB analysis using 67514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 67514-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67514-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67514-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 67514-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ZEB2 Monoclonal antibody (67514-1-Ig, Clone:1D3A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: K-562 cells, HT-1080 cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

67514-1-Ig targets ZEB2 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZEB2 fusion protein Ag25477
• Full Name: zinc finger E-box binding homeobox 2
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC127102
• Gene Symbol: ZEB2
• Gene ID (NCBI): 9839
• RRID: AB_2882735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Exp Cell Res; TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.; Authors - Longhui Ruan
human	WB	Exp Physiol; ZEB1 recruit Brg1 to regulate airway remodeling epithelial-mesenchymal transition in asthma.; Authors - Ting Wang
human	WB,IF	Int J Med Sci; Lnc NR2F1-AS1 Promotes Breast Cancer Metastasis by Targeting the MiR-25-3p/ZEB2 Axis; Authors - Duanyang Zhai ; KD Validated
human	WB	Cancer Gene Ther; AMIGO2 enhances the invasive potential of colorectal cancer by inducing EMT; Authors - Runa Izutsu