ZBTB7A Recombinant antibody, PBS Only

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  WB analysis using 83616-5-RR (same clone as 83616-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83616-5-RR (ZBTB7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83616-5-RR (same clone as 83616-5-PBS)

  WB result of ZBTB7A antibody (83616-5-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZBTB7A transfected HEK-293 cells. This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83616-5-RR (same clone as 83616-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83616-5-RR (ZBTB7A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83616-5-RR (same clone as 83616-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZBTB7A antibody (83616-5-RR, Clone: 240555A6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83616-5-RR (same clone as 83616-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZBTB7A antibody (83616-5-RR, Clone: 240555A6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83616-5-RR (same clone as 83616-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZBTB7A Recombinant antibody (83616-5-RR, Clone:240555A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83616-5-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ZBTB7A {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83616-5-PBS

Clone No.240555A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

HIV 1 1st binding protein 1, FBI-1, FBI1, FBI 1, Factor binding IST protein 1

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Product Information

83616-5-PBS targets ZBTB7A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc finger and BTB domain containing 7A
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa, 75-80 kDa
• GenBank Accession Number: BC113511
• Gene Symbol: ZBTB7A
• Gene ID (NCBI): 51341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O95365
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.