YY1 Recombinant antibody

YY1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2K12

Cat no : 82712-5-RR

Synonyms

NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA

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Validation Data Gallery

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  IF Staining of HepG2 using 82712-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 82712-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82712-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82712-5-RR against Human YY1 were performed. The affinity constant is 0.412 pM.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82712-5-RR targets YY1 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YY1 fusion protein Ag17792
• Full Name: YY1 transcription factor
• Calculated Molecular Weight: 414 aa, 45 kDa
• GenBank Accession Number: BC037308
• Gene Symbol: YY1
• Gene ID (NCBI): 7528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.