CoraLite® Plus 488-conjugated YTHDF3-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDF3-specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25537

Synonyms

FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3

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Validation Data Gallery

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  IF Staining of PC-12 using CL488-25537

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 YTHDF3-specific antibody (CL488-25537) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25537 targets YTHDF3-specific in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDF3-specific fusion protein Ag22374
• Full Name: YTH domain family, member 3
• Calculated Molecular Weight: 585 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC052970
• Gene Symbol: YTHDF3
• Gene ID (NCBI): 253943
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.