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  • KD/KO Validated

YTHDF3-specific Polyclonal antibody

YTHDF3-specific Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 25537-1-AP


YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25537-1-AP targets YTHDF3-specific in WB, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF3-specific fusion protein Ag22374
Full Name YTH domain family, member 3
Calculated Molecular Weight 585 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.


Product Specific Protocols
WB protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
IHC protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
IF protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
  • KD Validated

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
  • KD Validated

Adv Sci (Weinh)

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Authors - Yu Sun
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)