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YTHDF3 Recombinant antibody, PBS Only (Capture)

YTHDF3 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242149F3

Cat no : 84709-3-PBS

Synonyms

YTH domain-containing family protein 3, DF3, 242149F3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDF3 Recombinant antibody (84709-3-RR)

    WB analysis using 84709-3-RR (same clone as 84709-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84709-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01516-1

    Cytometric bead array standard curve of MP01516-1

    Cytometric bead array standard curve of MP01516-1, YTHDF3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84709-3-PBS. Detection antibody: 84709-1-PBS. Standard: Ag22407. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01516-2

    Cytometric bead array standard curve of MP01516-2

    Cytometric bead array standard curve of MP01516-2, YTHDF3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84709-3-PBS. Detection antibody: 84709-2-PBS. Standard: Ag22407. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84709-3-RR

    Affinity and Kinetic Characterization of 84709-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

Product Information

84709-3-PBS targets YTHDF3 as part of a matched antibody pair:

MP01516-1: 84709-3-PBS capture and 84709-1-PBS detection (validated in Cytometric bead array)

MP01516-2: 84709-3-PBS capture and 84709-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YTHDF3 fusion protein Ag22407
Full Name YTH domain family, member 3
Calculated Molecular Weight 585 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.