YTHDF3 Recombinant antibody

YTHDF3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242149F3

Cat no : 84709-3-RR

Synonyms

YTH domain-containing family protein 3, DF3, 242149F3

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Validation Data Gallery

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  WB analysis using 84709-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84709-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YTHDF3 fusion protein Ag22407
• Full Name: YTH domain family, member 3
• Calculated Molecular Weight: 585 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC052970
• Gene Symbol: YTHDF3
• Gene ID (NCBI): 253943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.