YTHDC1 Monoclonal antibody, PBS Only

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  WB analysis using 67911-1-Ig (same clone as 67911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

YTHDC1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67911-1-PBS

Clone No.1F5G11

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Product Information

67911-1-PBS targets YTHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: YTHDC1 fusion protein Ag6289
• Full Name: YTH domain containing 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: ~100 kDa
• GenBank Accession Number: BC053863
• Gene Symbol: YTHDC1
• Gene ID (NCBI): 91746
• RRID: AB_2918666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96MU7
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).