YTHDC1 Monoclonal antibody

YTHDC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F5G11

Reviews (2)

Cat no : 67911-1-Ig

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Validation Data Gallery

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  WB analysis using 67911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  IHC staining of mouse testis using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, MDA-MB-231 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67911-1-Ig targets YTHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: YTHDC1 fusion protein Ag6289
• Full Name: YTH domain containing 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: ~100 kDa
• GenBank Accession Number: BC053863
• Gene Symbol: YTHDC1
• Gene ID (NCBI): 91746
• RRID: AB_2918666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Publications

Species	Application	Title
human	WB	Mol Carcinog; m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma; Authors - Xiaoxin Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality with clear band.

• Applications: Western Blot
• Cell Tissue Type: HEK293T

FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was good. Did show clear band for YTHDC1 at around 100kDa for the western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: 293T

YTHDC1 Antibody Western Blot validation (1:4000 dilution) in 293T (Cat no:67911-1-Ig)