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CoraLite®594-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9A1

Cat no : CL594-66652

Synonyms

X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CoraLite®594-conjugated XRCC2 Monoclonal antibody (CL594-66652)

    IF Staining of L02 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated XRCC2 antibody (CL594-66652, Clone: 3D9A1 ) at dilution of 1:100, CL488-Phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated XRCC2 Monoclonal antibody (CL594-66652)

    IF Staining of HepG2 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66652 (XRCC2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated XRCC2 Monoclonal antibody (CL594-66652)

    FC experiment of HepG2 using CL594-66652

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC2 (CL594-66652, Clone:3D9A1) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inL02 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66652 targets XRCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC2 fusion protein Ag14421
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Calculated Molecular Weight 280 aa, 32 kDa
GenBank Accession NumberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2883610
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

Protocols

Product Specific Protocols
IF protocol for CL594 XRCC2 antibody CL594-66652Download protocol
FC protocol for CL594 XRCC2 antibody CL594-66652Download protocol
Standard Protocols
Click here to view our Standard Protocols