WTAP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

WTAP Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A10G9

Cat no : 60188-1-PBS

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

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Validation Data Gallery

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60188-1-PBS targets WTAP in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WTAP fusion protein Ag0259
• Full Name: Wilms tumor 1 associated protein
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000383
• Gene Symbol: WTAP
• Gene ID (NCBI): 9589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.