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  • KD/KO Validated

WTAP Monoclonal antibody

WTAP Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat and More (1)







Cat no : 60188-1-Ig


Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60188-1-Ig targets WTAP in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, drosophila
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259
Full Name Wilms tumor 1 associated protein
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC000383
Gene Symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.


Product Specific Protocols
WB protocol for WTAP antibody 60188-1-IgDownload protocol
IHC protocol for WTAP antibody 60188-1-IgDownload protocol
IF protocol for WTAP antibody 60188-1-IgDownload protocol
IP protocol for WTAP antibody 60188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Authors - Shay Geula

Nat Cell Biol

Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes

Authors - Dario F De Jesus

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
mouseIHC, IF

Nat Neurosci

Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.

Authors - Daria Merkurjev

Mol Cell

Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m 6 A Methylation

Authors - Hui-Lung Sun

Mol Cell

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Authors - Nandan S Gokhale


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Elaine (Verified Customer) (01-20-2021)

Antibody works fine in Western blot on MEF cells

  • Applications: Western Blot

Daniel (Verified Customer) (06-25-2019)

RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: RCC4 human cells
WTAP Antibody Western Blot, validation (1:2000 dilution) in RCC4 human cells (Cat no:60188-1-Ig)