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- KD/KO Validated
WTAP Polyclonal antibody
WTAP Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 10200-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, MOLT-4 cells, L02 cells, K-562 cells |
| Positive IP detected in | Jurkat cells, HEK-293 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 13 publications below |
| WB | See 51 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 4 publications below |
Product Information
10200-1-AP targets WTAP in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WTAP fusion protein Ag0259 |
| Full Name | Wilms tumor 1 associated protein |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC000383 |
| Gene Symbol | WTAP |
| Gene ID (NCBI) | 9589 |
| RRID | AB_2216349 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WTAP antibody 10200-1-AP | Download protocol |
| IHC protocol for WTAP antibody 10200-1-AP | Download protocol |
| IP protocol for WTAP antibody 10200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11 | ||
Nat Cell Biol The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA. | ||
Mol Cell Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.
| ||
Mol Cell Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal. |
