WNT3A Recombinant antibody, PBS Only

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  IF Staining of HepG2 using 84577-4-RR (same clone as 84577-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WNT3A antibody (84577-4-RR, Clone: 241993E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84577-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84577-4-RR (same clone as 84577-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug WNT3A Recombinant antibody (84577-4-RR, Clone:241993E8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84577-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84577-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84577-4-RR against Human WNT3A were performed. The affinity constant is 4.67 nM.

WNT3A {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84577-4-PBS

Clone No.241993E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Protein Wnt-3a, Protein Wnt 3a, 241993E8

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Product Information

84577-4-PBS targets WNT3A in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WNT3A fusion protein Ag25051
• Full Name: wingless-type MMTV integration site family, member 3A
• Calculated Molecular Weight: 352 aa, 39 kDa
• GenBank Accession Number: BC103921
• Gene Symbol: WNT3A
• Gene ID (NCBI): 89780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P56704
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.