WAPL Recombinant antibody

WAPL Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242443C1

Cat no : 84877-5-RR

Synonyms

WAPL/FOE, FOE, 242443C1, WAPL cohesin release factor, WAPAL

---

Validation Data Gallery

• 

  WB analysis using 84877-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84877-5-RR (WAPL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 84877-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 84877-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 84877-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL antibody (84877-5-RR, Clone: 242443C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84877-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84877-5-RR against Human WAPL were performed. The affinity constant is 0.23 nM.

---

Tested Applications

• Positive WB detected in: U2OS cells, MCF-7 cells, K-562 cells, HepG2 cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84877-5-RR targets WAPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WAPL fusion protein Ag9585
• Full Name: wings apart-like homolog (Drosophila)
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC017393
• Gene Symbol: WAPL
• Gene ID (NCBI): 23063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.