CoraLite®594-conjugated Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-26520

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

---

Validation Data Gallery

• 

  IF Staining of A549 using CL594-26520

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:400, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of HUVEC using CL594-26520

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: A549 cells, HUVEC cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-26520 is Vinculin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Vinculin fusion protein Ag24942
• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: VCL
• Gene ID (NCBI): 7414
• RRID: AB_2919877
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.