Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A11F8

Cat no : 66305-2-PBS

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

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Validation Data Gallery

• 

  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

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Product Information

66305-2-PBS targets Vinculin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vinculin fusion protein Ag24946
• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: Vinculin
• Gene ID (NCBI): 7414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.