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Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A11F8

Cat no : 66305-2-Ig

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMKN-45 cells, HUVEC cells, LNCaP cells, K-562 cells, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells, HGC-27 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66305-2-Ig targets Vinculin in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946
Full Name vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight124 kDa
GenBank Accession NumberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 66305-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols