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VPS4B Monoclonal antibody, PBS Only

VPS4B Monoclonal Antibody for WB, IP, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3G7

Cat no : 68443-1-PBS

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4B Monoclonal antibody (68443-1-Ig)

    WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using VPS4B Monoclonal antibody (68443-1-Ig)

    WB analysis of NIH/3T3 using 68443-1-Ig (same clone as 68443-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using VPS4B Monoclonal antibody (68443-1-Ig)

    IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS4B Monoclonal antibody (68443-1-Ig)

    IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68443-1-PBS targets VPS4B in WB, IP, IF, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS4B fusion protein Ag11610
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated Molecular Weight 444 aa, 49 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC039574
Gene Symbol VPS4B
Gene ID (NCBI) 9525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)