Tested Applications
Positive WB detected in | LNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68443-1-Ig targets VPS4B in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.
Tested Reactivity | Human, mouse , rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | VPS4B fusion protein Ag11610 Predict reactive species |
Full Name | vacuolar protein sorting 4 homolog B (S. cerevisiae) |
Calculated Molecular Weight | 444 aa, 49 kDa |
Observed Molecular Weight | ~50 kDa |
GenBank Accession Number | BC039574 |
Gene Symbol | VPS4B |
Gene ID (NCBI) | 9525 |
RRID | AB_3085156 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O75351 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)
Protocols
Product Specific Protocols | |
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WB protocol for VPS4B antibody 68443-1-Ig | Download protocol |
IF protocol for VPS4B antibody 68443-1-Ig | Download protocol |
IP protocol for VPS4B antibody 68443-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |