CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55491

Synonyms

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-55491

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL488-55491

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Calculated Molecular Weight: 62 kDa
• GenBank Accession Number: NM_020309
• Gene Symbol: VGLUT1
• Gene ID (NCBI): 57030
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.