VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 55491-1-AP

Synonyms

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI

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Validation Data Gallery

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  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 55491-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 55491-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human VGLUT1 (55491-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: rat brain tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 13 publications below

Product Information

55491-1-AP targets VGLUT1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Calculated Molecular Weight: 62 kDa
• GenBank Accession Number: NM_020309
• Gene Symbol: VGLUT1
• Gene ID (NCBI): 57030
• RRID: AB_2881348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	PLoS Biol; The anterior cingulate cortex controls the hyperactivity in subthalamic neurons in male mice with comorbid chronic pain and depression; Authors - Ying-Di Wang
human	IF	iScience; Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons; Authors - Waka Lin
human	IF	Neurobiol Dis; GRN-/- iPSC-derived cortical neurons recapitulate the pathological findings of both frontotemporal lobar degeneration and neuronal ceroidolipofuscinosis; Authors - Patrizia Bossolasco
mouse	IF	J Neurosci; Insula→Amygdala and Insula→Thalamus Pathways Are Involved in Comorbid Chronic Pain and Depression-Like Behavior in Mice; Authors - Jing Chen
rat	IF	Neurobiol Dis; PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by influencing synaptic function in the primary motor cortex of rats.; Authors - Juan Mo
human	IF	Front Cell Infect Microbiol; Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons.; Authors - Hironori Bando