VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55491-1-AP

Synonyms

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI



Tested Applications

Positive IHC detected inrat brain tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55491-1-AP targets VGLUT1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Calculated Molecular Weight 62 kDa
GenBank Accession NumberNM_020309
Gene Symbol VGLUT1
Gene ID (NCBI) 57030
RRIDAB_2881348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for VGLUT1 antibody 55491-1-APDownload protocol
IF protocol for VGLUT1 antibody 55491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PLoS Biol

The anterior cingulate cortex controls the hyperactivity in subthalamic neurons in male mice with comorbid chronic pain and depression

Authors - Ying-Di Wang
humanIF

iScience

Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons

Authors - Waka Lin
humanIF

Neurobiol Dis

GRN-/- iPSC-derived cortical neurons recapitulate the pathological findings of both frontotemporal lobar degeneration and neuronal ceroidolipofuscinosis

Authors - Patrizia Bossolasco
ratIF

Neurobiol Dis

PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by influencing synaptic function in the primary motor cortex of rats.

Authors - Juan Mo
humanIF

Front Cell Infect Microbiol

Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons.

Authors - Hironori Bando
mouseIF

Exp Anim

TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models

Authors - Haiqing Zhang