VDAC2 Recombinant antibody, PBS Only (Capture)

VDAC2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241569G11

Cat no : 84225-2-PBS

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

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Validation Data Gallery

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  WB analysis using 84225-2-RR (same clone as 84225-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84225-2-RR (same clone as 84225-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01162-1

  Cytometric bead array standard curve of MP01162-1, VDAC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84225-2-PBS. Detection antibody: 84225-1-PBS. Standard: Ag2266. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 84225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

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Product Information

84225-2-PBS targets VDAC2 as part of a matched antibody pair:

MP01162-1: 84225-2-PBS capture and 84225-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VDAC2 fusion protein Ag2266
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 294 aa, 32 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: BC000165
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)