VDAC2 Recombinant antibody

VDAC2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241569G11

Cat no : 84225-2-RR

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569G11

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Validation Data Gallery

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  WB analysis using 84225-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84225-2-RR (VDAC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 84225-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-2-RR, Clone:241569G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84225-2-RR against Human VDAC2 were performed. The affinity constant is 0.48 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HuH-7 cells, L02 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84225-2-RR is VDAC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VDAC2 fusion protein Ag2266
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 294 aa, 32 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: BC000165
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)