Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HuH-7 cells, L02 cells, mouse brain tissue, mouse heart tissue, rat brain tissue |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84225-2-RR targets VDAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | VDAC2 fusion protein Ag2266 Predict reactive species |
Full Name | voltage-dependent anion channel 2 |
Calculated Molecular Weight | 294 aa, 32 kDa |
Observed Molecular Weight | 31-33 kDa |
GenBank Accession Number | BC000165 |
Gene Symbol | VDAC2 |
Gene ID (NCBI) | 7417 |
RRID | AB_3671778 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P45880 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)
Protocols
Product Specific Protocols | |
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WB protocol for VDAC2 antibody 84225-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |