Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
This antibody is used as the isotype control of mouse IgG3. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
WB protocol for Mouse IgG3 isotype control antibody 66360-4-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
"Mouse IgG3 isotype control antibodies" comparison
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Proteintech
Mouse IgG3 isotype control Monoclonal antibody
Catalog Number
66360-4-Ig
Citations
-
Dilutions
WB : 1:1000-1:4000
Applications
WB, ELISA
Reactivity
megathura crenulata
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
