CoraLite® Plus 488-conjugated UTP14A Polyclonal antibody

• Featured Product
• KD/KO Validated

UTP14A Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11474

Synonyms

U3 small nucleolar RNA-associated protein 14 homolog A, SDCCAG16, NY CO 16, KIAA0266, Antigen NY-CO-16

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-11474

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 UTP14A antibody (CL488-11474) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HepG2 using CL488-11474

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Utp14a Polyclonal Antibody (CL488-11474)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11474 targets UTP14A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UTP14A fusion protein Ag2027
• Full Name: UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast)
• Calculated Molecular Weight: 771 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC014987
• Gene Symbol: UTP14A
• Gene ID (NCBI): 10813
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.