USP14 Recombinant antibody, PBS Only

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  WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

  WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

  IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82128-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

USP14 Uni-rAb^TM Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 82128-1-PBS

Clone No.3G12

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

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Product Information

82128-1-PBS targets USP14 in WB, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP14 fusion protein Ag5979
• Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003556
• Gene Symbol: USP14
• Gene ID (NCBI): 9097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54578
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.