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USP14 Recombinant antibody

USP14 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3G12

Cat no : 82128-1-RR

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP14 Recombinant antibody (82128-1-RR)

    WB analysis using 82128-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using USP14 Recombinant antibody (82128-1-RR)

    WB analysis of HeLa using 82128-1-RR

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using USP14 Recombinant antibody (82128-1-RR)

    IP experiment of mouse liver using 82128-1-RR

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug.

  • Affinity and Kinetic Characterization of 82128-1-RR

    Affinity and Kinetic Characterization of 82128-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293T cells, DU 145 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82128-1-RR targets USP14 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen USP14 fusion protein Ag5979
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

Protocols

Product Specific Protocols
WB protocol for USP14 antibody 82128-1-RRDownload protocol
IP protocol for USP14 antibody 82128-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols