USP14 Recombinant antibody

• Featured Product
• KD/KO Validated

USP14 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3G12

Cat no : 82128-1-RR

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

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Validation Data Gallery

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  WB analysis using 82128-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82128-1-RR

  WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

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  IP experiment of mouse liver using 82128-1-RR

  IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug.

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  Affinity and Kinetic Characterization of 82128-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, HEK-293T cells, DU 145 cells, Jurkat cells, NIH/3T3 cells, C6 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82128-1-RR targets USP14 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP14 fusion protein Ag5979
• Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003556
• Gene Symbol: USP14
• Gene ID (NCBI): 9097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.