UPF1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

UPF1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B6B5

Cat no : 66898-1-PBS

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

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Validation Data Gallery

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  WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66898-1-Ig (same clone as 66898-1-PBS)

  WB result of UPF1 antibody (66898-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using UPF1 antibody (66898-1-Ig, Clone: 3B6B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human UPF1 (66898-1-Ig, Clone:3B6B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66898-1-PBS targets UPF1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UPF1 fusion protein Ag28320
• Full Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 123-130 kDa
• GenBank Accession Number: BC039817
• Gene Symbol: UPF1
• Gene ID (NCBI): 5976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.