UHRF1 Recombinant antibody, PBS Only

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  WB analysis using 84489-5-RR (same clone as 84489-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84489-5-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84489-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84489-5-RR (same clone as 84489-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-5-RR, Clone: 241872G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84489-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84489-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84489-5-RR against Human UHRF1 were performed. The affinity constant is 0.842 nM.

UHRF1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84489-5-PBS

Clone No.241872G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HuNp95, hUHRF1, hNp95, EC:2.3.2.27, E3 ubiquitin-protein ligase UHRF1

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Product Information

84489-5-PBS targets UHRF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UHRF1 fusion protein Ag17498
• Full Name: ubiquitin-like with PHD and ring finger domains 1
• Calculated Molecular Weight: 806 aa, 91 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC113875
• Gene Symbol: UHRF1
• Gene ID (NCBI): 29128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96T88
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).