CoraLite®594-conjugated UCH-L1/PGP9.5 Polyclonal antibody

• Featured Product
• KD/KO Validated

UCH-L1/PGP9.5 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-14730

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP9/5, PGP 9.5

---

Validation Data Gallery

• 

  IF Staining of mouse brain using CL594-14730

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 UCHL1/PGP9.5 antibody (CL594-14730) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-14730

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 UCHL1/PGP9.5 antibody (CL594-14730) at dilution of 1:200.

• 

  FC experiment of Y79 using CL594-14730

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human UCHL1/PGP9.5 (CL594-14730) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: Y79 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-14730 targets UCH-L1/PGP9.5 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6490
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000332
• Gene Symbol: UCHL1
• Gene ID (NCBI): 7345
• RRID: AB_2919814
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Publications

Species	Application	Title
	IF	J Neuroinflammation; CX3CR1+/UCHL1+ microglial extracellular vesicles in blood: a potential biomarker for multiple sclerosis; Authors - Jing Duan

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C