UCH-L1/PGP9.5 Polyclonal antibody

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  WB analysis using 14730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14730-1-AP (UCHL1/PGP9.5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14730-1-AP (UCHL1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 14730-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14730-1-AP (UCHL1/PGP9.5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 14730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 14730-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C6 using 14730-1-AP

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human UCHL1/PGP9.5 (14730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

UCH-L1/PGP9.5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 14730-1-AP

Publications(128)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP9/5, PGP 9.5

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Tested Applications

• Positive WB detected in: A549 cells, NCI-H1299 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: A549 cells, mouse brain tissue
• Positive FC (Intra) detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 27 publications below
• IHC: See 29 publications below
• IF: See 69 publications below
• ELISA: See 2 publications below
• CoIP: See 5 publications below

Product Information

14730-1-AP targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, monkey, non-human primate
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6490
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000332
• Gene Symbol: UCHL1
• Gene ID (NCBI): 7345
• RRID: AB_2210497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09936
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Publications

Species	Application	Title
mouse	IHC	Nature; The gut metabolite indole-3 propionate promotes nerve regeneration and repair.; Authors - Elisabeth Serger
mouse	IF	Nat Biotechnol; Whole-body cellular mapping in mouse using standard IgG antibodies; Authors - Hongcheng Mai
mouse	WB	Nature; A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.; Authors - Putianqi Wang
mouse	IHC	Cell Metab; The circadian clock time tunes axonal regeneration; Authors - Francesco De Virgiliis
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Cell Metab; Metabolic stress drives sympathetic neuropathy within the liver.; Authors - Kaili Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaolu (Verified Customer) (06-05-2024)

This antibody works very well on WB. I tried dilution and found that 1:25000 is good enough. Do not reduced the dilution, or you will get a very strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Rat cell and Human cell

UCH-L1/PGP9.5 Antibody Western Blot validation (1:25000 dilution) in Rat cell and Human cell (Cat no:14730-1-AP)

FH

Dan (Verified Customer) (10-30-2023)

This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Breast cancer and colorectal cancer cell lines

UCH-L1/PGP9.5 Antibody Western Blot validation (1:2000 dilution) in Breast cancer and colorectal cancer cell lines (Cat no:14730-1-AP)

FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse ovary

UCH-L1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)

FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: paw skin

UCH-L1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)

FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Brain Homogenate