UCHL1/PGP9.5 Polyclonal antibody

• Featured Product
• KD/KO Validated

UCHL1/PGP9.5 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

90

Reviews (3)

Cat no : 14730-1-AP

Synonyms

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1, UCHL1/PGP9.5

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Validation Data Gallery

• 

  WB analysis using 14730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14730-1-AP (UCHL1/PGP9.5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 14730-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C6 using 14730-1-AP

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human UCHL1/PGP9.5 (14730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, NCI-H1299 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IF detected in: A549 cells, mouse brain tissue
• Positive FC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 20 publications below
• IF: See 45 publications below
• ELISA: See 2 publications below
• CoIP: See 3 publications below

Product Information

14730-1-AP targets UCHL1/PGP9.5 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, pig, Non-human primate
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCHL1/PGP9.5 fusion protein Ag6490
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 27 kDa
• GenBank accession number: BC000332
• Gene symbol: UCHL1
• Gene ID (NCBI): 7345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Publications

Species	Application	Title
mouse	IHC	Nature; The gut metabolite indole-3 propionate promotes nerve regeneration and repair.; Authors - Elisabeth Serger
mouse	WB	Nature; A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.; Authors - Putianqi Wang
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Cell Metab; Metabolic stress drives sympathetic neuropathy within the liver.; Authors - Kaili Liu
mouse	WB,IF	Cell Metab; Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue.; Authors - Haochen Jiang
mouse	WB	Acta Neuropathol; Changes in proteome solubility indicate widespread proteostatic disruption in mouse models of neurodegenerative disease.; Authors - Michael C Pace

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse ovary

UCHL1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)

FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: paw skin

UCHL1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)

FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Brain Homogenate

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