UBE2K Polyclonal antibody, PBS Only

UBE2K Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11834-3-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11834-3-AP (same clone as 11834-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 11834-3-AP (same clone as 11834-3-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11834-3-AP (same clone as 11834-3-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11834-3-AP (same clone as 11834-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11834-3-AP (UBE2K antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 11834-3-AP (same clone as 11834-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 11834-3-AP (same clone as 11834-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11834-3-PBS in a different storage buffer formulation.

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Product Information

11834-3-PBS targets UBE2K in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2K fusion protein Ag2415
• Full Name: ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• Calculated Molecular Weight: 200 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC022804
• Gene Symbol: UBE2K
• Gene ID (NCBI): 3093
• RRID: AB_2241168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.