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UBE2K Polyclonal antibody

UBE2K Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11834-3-AP

Synonyms

E2-25K, HIP 2, HIP2, HYPG, LIG, UBE2K, Ubiquitin carrier protein, Ubiquitin protein ligase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2K Polyclonal antibody (11834-3-AP)

    WB analysis using 11834-3-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using UBE2K Polyclonal antibody (11834-3-AP)

    WB analysis of mouse testis using 11834-3-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using UBE2K Polyclonal antibody (11834-3-AP)

    WB analysis of human brain using 11834-3-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using UBE2K Polyclonal antibody (11834-3-AP)

    IHC staining of human gliomas using 11834-3-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11834-3-AP (UBE2K antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using UBE2K Polyclonal antibody (11834-3-AP)

    IHC staining of human thyroid cancer using 11834-3-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using UBE2K Polyclonal antibody (11834-3-AP)

    IHC staining of human thyroid cancer using 11834-3-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, human brain tissue, rat brain tissue, rat testis tissue
Positive IHC detected inhuman gliomas tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

11834-3-AP targets UBE2K in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2K fusion protein Ag2415
Full Name ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
Calculated Molecular Weight 200 aa, 22 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC022804
Gene Symbol UBE2K
Gene ID (NCBI) 3093
RRIDAB_2241168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2K antibody 11834-3-APDownload protocol
IHC protocol for UBE2K antibody 11834-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Oncol

UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma

Authors - Wen Fu
View Article
humanWB

Biochem Biophys Res Commun

Ixazomib inhibits myeloma cell proliferation by targeting UBE2K.

Authors - Qingqing Wang
View Article
humanWB

Biomed Environ Sci

Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells.

Authors - Mo Juan Li
View Article
ratWB

Neuropathology

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

Authors - Kazuhiko Watabe
View Article