Tubulin Beta Class V Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66362-1-Ig (same clone as 66362-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66362-1-Ig (same clone as 66362-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66362-1-Ig (same clone as 66362-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

Tubulin Beta Class V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66362-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, Canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

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Product Information

66362-1-PBS targets Tubulin Beta Class V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, Canine samples.

• Tested Reactivity: human, mouse, Canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulin, beta 6
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_032525
• Gene Symbol: Beta Tubulin
• Gene ID (NCBI): 84617
• RRID: AB_2881742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.