CoraLite®555-conjugated Tom22 Monoclonal antibody

Tom22 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1C2B11

Cat no : CL555-66562

Synonyms

TOMM22, MSTP065, MST065, hTom22, 1C9 2

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-66562

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 Tom22 antibody (CL555-66562, Clone: 1C2B11 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL555-66562

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated Tom22 Monoclonal antibody (CL555-66562, Clone:1C2B11)(red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2a isotype control (11A1B2) (CL555-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66562 targets Tom22 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tom22 fusion protein Ag1797
• Full Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009363
• Gene Symbol: TOMM22/Tom22
• Gene ID (NCBI): 56993
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).