Tom22 Monoclonal antibody

Tom22 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1C2B11

Cat no : 66562-1-Ig

Synonyms

TOMM22, MSTP065, MST065, hTom22, 1C9 2

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Validation Data Gallery

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  WB analysis using 66562-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66562-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66562-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66562-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tom22 antibody (66562-1-Ig, Clone: 1C2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

• 

  FC experiment of HEK-293 using 66562-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Tom22 (66562-1-Ig, Clone:1C2B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, T-47D cells, L02 cells, Raji cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66562-1-Ig targets Tom22 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tom22 fusion protein Ag1797
• Full Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009363
• Gene Symbol: TOMM22/Tom22
• Gene ID (NCBI): 56993
• RRID: AB_2881923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Publications

Species	Application	Title
human	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
mouse	IF	Nat Commun; Neutrophils restrain sepsis associated coagulopathy via extracellular vesicles carrying superoxide dismutase 2 in a murine model of lipopolysaccharide induced sepsis; Authors - Wenjie Bao

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C