CoraLite® Plus 488-conjugated Thyroglobulin Monoclonal antibody

Thyroglobulin Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4D3C5

Cat no : CL488-60272

Synonyms

TG, 4D3C5

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Validation Data Gallery

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  IF Staining of human thyroid cancer using CL488-60272

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite® Plus 488 Thyroglobulin antibody (CL488-60272, Clone: 4D3C5 ) at dilution of 1:200.

• 

  IF Staining of human thyroid cancer using CL488-60272

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite® Plus 488 Thyroglobulin antibody (CL488-60272, Clone: 4D3C5 ) at dilution of 1:200.

• 

  IF Staining of human thyroid cancer using CL488-60272

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using CoraLite® Plus 488 Thyroglobulin antibody (CL488-60272, Clone: 4D3C5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: human thyroid cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60272 targets Thyroglobulin in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thyroglobulin fusion protein Ag18070
• Full Name: thyroglobulin
• Calculated Molecular Weight: 2768 aa, 305 kDa
• GenBank Accession Number: BC140933
• Gene Symbol: Thyroglobulin
• Gene ID (NCBI): 7038
• RRID: AB_2919242
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.