Thyroglobulin Monoclonal antibody
Thyroglobulin Monoclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
4D3C5
Cat no : 60272-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human thyroid tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human thyroid cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
60272-1-Ig targets Thyroglobulin in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Thyroglobulin fusion protein Ag18070 |
| Full Name | thyroglobulin |
| Calculated Molecular Weight | 2768 aa, 305 kDa |
| GenBank Accession Number | BC140933 |
| Gene Symbol | Thyroglobulin |
| Gene ID (NCBI) | 7038 |
| RRID | AB_2881392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Thyroglobulin antibody 60272-1-Ig | Download protocol |
| IF protocol for Thyroglobulin antibody 60272-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Endocrinol Metab Identification of Eukaryotic Translation Initiation Factor 4B as a Novel Candidate Gene for Congenital Hypothyroidism | ||
Front Immunol Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto's Thyroiditis. | ||
Cancer Cell Int Identification of an immune-related signature indicating the dedifferentiation of thyroid cells. |
