CoraLite®594-conjugated TXNRD1 Monoclonal antibody

TXNRD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

1B10C4

Cat no : CL594-67728

Synonyms

TRXR1, GRIM 12, Gene associated with retinoic and interferon-induced mortality 12 protein, Gene associated with retinoic and IFN-induced mortality 12 protein, EC:1.8.1.9

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67728

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TXNRD1 antibody (CL594-67728, Clone: 1B10C4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67728

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TXNRD1 (CL594-67728, Clone:1B10C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-67728 targets TXNRD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TXNRD1 fusion protein Ag30071
• Full Name: thioredoxin reductase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC018122
• Gene Symbol: TXNRD1
• Gene ID (NCBI): 7296
• RRID: AB_2920175
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Publications

Species	Application	Title
human	IF	Chem Biol Interact; Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer; Authors - Ming-Shou Hsieh