TXNDC5 Recombinant antibody, PBS Only

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  WB analysis using 84756-5-RR (same clone as 84756-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84756-5-RR (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84756-5-RR (same clone as 84756-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (84756-5-RR, Clone: 242195G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84756-5-RR (same clone as 84756-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TXNDC5 Recombinant antibody (84756-5-RR, Clone:242195G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84756-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84756-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84756-5-RR against Human TXNDC5 were performed. The affinity constant is 2.31 nM.

TXNDC5 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84756-5-PBS

Clone No.242195G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-

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Product Information

84756-5-PBS targets TXNDC5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TXNDC5 fusion protein Ag13965
• Full Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001199
• Gene Symbol: TXNDC5
• Gene ID (NCBI): 81567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8NBS9
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)