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TXNDC5 Recombinant antibody

TXNDC5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242195G7

Cat no : 84756-5-RR

Synonyms

Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC5 Recombinant antibody (84756-5-RR)

    WB analysis using 84756-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84756-5-RR (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TXNDC5 Recombinant antibody (84756-5-RR)

    IHC staining of human stomach cancer using 84756-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TXNDC5 Recombinant antibody (84756-5-RR)

    IHC staining of human stomach cancer using 84756-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84756-5-RR (TXNDC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXNDC5 Recombinant antibody (84756-5-RR)

    IF Staining of HeLa using 84756-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (84756-5-RR, Clone: 242195G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84756-5-RR

    Affinity and Kinetic Characterization of 84756-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84756-5-RR against Human TXNDC5 were performed. The affinity constant is 2.31 nM.

Tested Applications

Positive WB detected inHepG2 cells, TF-1 cells, COLO 320 cells, K-562 cells, LNCap cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84756-5-RR targets TXNDC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TXNDC5 fusion protein Ag13965
Full Name thioredoxin domain containing 5 (endoplasmic reticulum)
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC001199
Gene Symbol TXNDC5
Gene ID (NCBI) 81567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)

Protocols

Product Specific Protocols
WB protocol for TXNDC5 antibody 84756-5-RRDownload protocol
IHC protocol for TXNDC5 antibody 84756-5-RRDownload protocol
IF protocol for TXNDC5 antibody 84756-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols