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CoraLite® Plus 488-conjugated TUFM Monoclonal antibody

TUFM Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A10D2

Cat no : CL488-67802

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TUFM Monoclonal anti (CL488-67802)

    IF Staining of HepG2 using CL488-67802

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TUFM antibody (CL488-67802, Clone: 2A10D2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TUFM Monoclonal anti (CL488-67802)

    FC experiment of HepG2 using CL488-67802

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TUFM (CL488-67802, Clone:2A10D2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67802 targets TUFM in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TUFM fusion protein Ag24976
Full Name Tu translation elongation factor, mitochondrial
Calculated Molecular Weight 50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TUFM antibody CL488-67802Download protocol
FC protocol for CL Plus 488 TUFM antibody CL488-67802Download protocol
Standard Protocols
Click here to view our Standard Protocols