TUFM Monoclonal antibody

TUFM Monoclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2A10D2

Reviews (1)

Cat no : 67802-1-Ig

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

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Validation Data Gallery

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  WB analysis using 67802-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67802-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 67802-1-Ig

  IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

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  IHC staining of human colon cancer using 67802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67802-1-Ig targets TUFM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TUFM fusion protein Ag24976
• Full Name: Tu translation elongation factor, mitochondrial
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC010041
• Gene Symbol: TUFM
• Gene ID (NCBI): 7284
• RRID: AB_2918565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (02-19-2023)

Works very Well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human pulmonary artery smooth muscle cells