CoraLite®594-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Mouse / IgG1


human, mouse, rat


WB, IHC, IF, FC (Intra)


CoraLite®594 Fluorescent Dye



Cat no : CL594-66375


beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3, tuj1, tuj1 antibody

Tested Applications

Positive WB detected inNeuro-2a cells
Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66375 targets TUBB3-specific/TUJ1 in WB, IHC, IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated Molecular Weight 55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.


Product Specific Protocols
WB protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375Download protocol
IF protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375Download protocol
FC protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375Download protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Rep

Sexual dimorphic impacts of systemic vincristine on lower urinary tract function.

Authors - Nao Iguchi

Exp Cell Res

A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development.

Authors - Yuki Shindo


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Victoria (Verified Customer) (10-15-2021)

Good IF detection of differentiating neurons in tissue at a concentration of 1:100.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: E3 chick neural tube. FA fixed.